NeoProteomics

Expertise. Innovation. Results.

ProtMapMSTM


Neo employs proprietary methods to help manage workflows and has developed ProtMapMSTM to analyze data that is generated by highly-sensitive machines which greatly accelerates research and interpretation.

ProtMapMSTM is a proprietary software tool intended to assist in the automated structural analysis of biologic based drugs, including quality control assessment of primary structure modifications and higher-order structure analysis using covalent labeling.

For structural analysis by oxidative labeling, ProtMapMSTM analyzes the readouts from labeled residues in the mass spectrometric analysis, providing information for mapping the protein surface and defining the solvent accessibility for residues of interest.

ProtMapMSTM integrates into covalent labeling workflow providing automated functionality for:

  • Date Format Conversion
  • Mass Spectrum Interpretation
  • Detection and verification of all peptide species
  • Confirmation of site of peptide modification
  • Quantification of the extent of peptide label as a function of covalent label exposure

ProtMapMSTM:

  • Eliminates a major bottleneck in structural mass spectrometry experiments for Biologics Characterization
  • Removes bias from manual calculations
In a single experiment, benchmarking shows a 48 person-hour time differential between expert manual methods and ProtMapMSTM analyzing the same data!

Input interface for ProtMapMSTM Version 2.5.0.25

Integrated algorithms for high-throughput examination of covalently labeled biomolecules by structural mass spectrometry. Kaur P, Kiselar JG, Chance MR. Anal Chem. 2009 Oct 1;81(19):8141-9.
 
Typical output generated using ProtMapMSTM. Dose response plot is seen in the top left. The corresponding selected ion chromatograms of peptide isoforms across multiple hydroxyl radical exposure are seen on the right.

Integrated algorithms for high-throughput examination of covalently labeled biomolecules by structural mass spectrometry. Kaur P, Kiselar JG, Chance MR. Anal Chem. 2009 Oct 1;81(19):8141-9.