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Comparison of Biophysical Techniques


Contact our NeoProteomics' experts to help determine

which techniques would be best for you!

Although X-ray crystallography is the best overall solution to determine an epitope, it may not always be feasible. This chart highlights popular methods offering a variety of solutions.
 

X-Ray

NMR

Peptide
Microarrays

SMS

Cost

High

High

Low

Moderate

Throughput

Low

Low

High

Medium

Technical challenges

Crystals may not be formed

Limited to proteins of up to medium size (<30 kda="" span="">

Limited to short peptide lengths
(
<15 kda="" span="">

Requires specialized equipment

State

Crystal

Solution

Solution

Solution

Type

Linear and conformational

Linear and conformational

Linear

Linear and conformational


 
Hydroxyl Radical Footprinting vs. HD Exchange

HDX

Hydroxyl Radical Footprinting

Target probes

Back bone hydrogens

Amino acid side chains

Protease

Pepsin

No limitation

Stability

Reversible covalent changes

Stable, covalent modifications

Timescale

Seconds to hours

Microseconds to Seconds

Number of probes

19

16

Risk

Back exchange

Perturbation due to excessive oxidation

Membrane proteins

Challenging, early results

Established

Spatial resolution level

Peptide, challenging to get to residue level

Residue level

Size

Limitations due to pepsin/time

No limitation